Sex identification of turkey embryos using a multiplex polymerase chain reaction
نویسندگان
چکیده
منابع مشابه
Identification of co-occurring Branchinecta fairy shrimp species from encysted embryos using multiplex polymerase chain reaction.
Morphological identification of many fairy shrimp species is difficult because distinguishing characters are restricted to adults. We developed two multiplex polymerase chain reaction assays that differentiate among three Branchinecta fairy shrimp with distributional overlap in southern California vernal pools. Two of the species are federally listed as threatened. Molecular identification of B...
متن کاملIdentification of Dermatophytes Using Multiplex Polymerase Chain Reaction
BACKGROUND Multiplex polymerase chain reaction (PCR) allows more than two target DNA molecules to be amplified with more than two primers. This method is also useful for detecting various other organisms simultaneously within a single test tube, and the scope of its use has been expanding widely in the field of clinical microbiology in recent years. OBJECTIVE To assess the value of multiplex ...
متن کاملDevelopment of a Multiplex Polymerase Chain Reaction for Differential Diagnosis of Canary Pox Virus
Background and Aims: A multiplex transcription-polymerase chain reaction (m-PCR) was developed for direct detection and discrimination between canarypox virus (CPV) and other avian poxvirus (APV). Materials and Methods: Three compatible primer sets were designed for m-PCR amplification of different loci fpv126, fpv140, and fpv167 located at highly conserved APV genes. Results: Results showed th...
متن کاملDetermination of Sperm Sex Ratio in Bovine Semen Using Multiplex Real-time Polymerase Chain Reaction
Gender selection is important in livestock industries; for example, female calves are required in the dairy industry. Sex-sorted semen is commonly used for the production of calves of the desired gender. However, assessment of the sex ratio of the sorted semen is tedious and expensive. In this study, a rapid, cost effective and reliable method for determining the sex ratio was developed using a...
متن کاملRfbo157 by Multiplex Polymerase Chain Reaction
A rapid method for detection of Escherichia coli O157: H7 using multiplex PCR was developed. Two oligonucleotide primer pairs were used for simultaneously detection of vt encoding verotoxin genes for virulence factor and rfbO157 encoding the O-antigen specific for E. coli O157: H7. Multiplex PCR generated two products of 215 bp and 420 bp for vt and rfbO157, respectively. Multiplex PCR detected...
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ژورنال
عنوان ژورنال: Poultry Science
سال: 1998
ISSN: 0032-5791
DOI: 10.1093/ps/77.5.718